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5 Conclusion Therefore, the mitochondrial dysfunction observed in aging is accompanied by the intracellular GSH/GSSG imbalance, a decrease of the H 2 S content in organelles and the expression levels of UCP3 , as well as the uncoupling of mtNOS with an increase in ROS production, disruption of Ca 2+ homeostasis, etc., which leads to the induction of mPTP with high conductance and causing cardiac dysfunction

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Precursor ions were filtered with a minimum intensity threshold of 2.5e 4 , precursor fit error of 50%, precursor fit window of 1.1, and dynamic exclusion time of 60 s after a fragmentation event

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