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If they start to dip, it might be time to increase their frequency for a bit
Cell viability assay For cell viability assays, FASN +/+ -PyMT MEFs and HC11 cells were seeded (5 10 3 cells/well) in 96-well plates for 24 h and subsequently replaced with fresh medium with vehicle (control) or increasing concentration of sodium citrate (Sigma) (1, 2.5, 5.0, and 10 mM) or SB204990 (Tocris Bioscience) (10, 20, 30, 40, 60, and 100 M)
The metals recirculate and are free to do more damage
