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To determine whether GPI-MFI2 co-localizes with caveolae or clathrin-coated pits, we employed a modified internalization assay previously used in measuring the endocytosis of other membrane receptors [49]

Starting with a low dose and increasing it gradually can minimize side effects

The fluorescence decay measured from the eumelanin-enriched dark brown hair, dark pigmented HCM, the pheomelanin-enriched red hair and light pigmented HCM were fitted using a two exponentials model for comparison (Supplementary Table 5)