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24 h later, culture media were replaced with 1 mL of serum-free DMEM containing 50 ng/mL of recombinant hTNF- (Peprotech) and cultured for a further 24 h before collecting cells and supernatants
PubMed Central, Liang, Chen, et al
Kilmer McCully, an American pathologist, proposed the homocysteine hypothesis of arteriosclerosis
As ROS are a known inhibitor of mTOR activation, they showed that intracellular GSH was able to buffer ROS leading to mTOR activation, metabolic reprogramming, and resultant inflammatory response of T cells in vitro and in vivo (45)
