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Finally, translational studies should focus on establishing robust in vitroin vivo correlations that link regional deposition to brain exposure and pharmacodynamic outcomes, enabling rational dose selection and improved predictability across patient populations

Figueroa et al., 2017

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Fifty microliters of standard solutions were mixed with assay cocktail (12.5 l of 4 mM NADPH in 5% NaHCO 3, 25 l of GSH reductase solution (Roche, No.105678, 5 mg/ml), 12.5 l of 10 mM 5,5-dithiobis-2-nitrobenzoic acid in 0.1 M phosphate buffer, 75 l MES buffer, 25 l distilled water) on 96-well plate