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After 24 hours from the first trial, a second trial is performed by placing the animal in the same container, but for 5 minutes
Bio Ind 4:189197 Ohtake Y, Watanabe K, Tezuka H, Ogata T, Yabuuchi S, Murata K, Kimura A (1988) The expression of gamma-glutamylcysteine synthetase gene of Escherichia coli in Saccharomyces cerevisiae
Therefore, cellular availability of GSH through glutathione synthesis is a critical determinant of sensitivity to ferroptosis
