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For a larger area, a bit more serum, added as needed to keep pace with the area being covered, gives more even coverage than stretching a fixed volume too thin

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The standard reconstitution protocol uses 2mL bacteriostatic water per 50mg vial, yielding 25mg/mL concentration, but some researchers use 1mL to create 50mg/mL solutions for smaller injection volumes

Cyanocobalamin is the most widely used form of Vitamin B12, and has hematopoietic activity apparently identical to that of the antianemia factor in purified liver extract