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The presence or absence of the Ospecific chain determines the appearance, smooth or rough, of a bacterial colony, and the rough type lipopolysaccharides lack the Ospecific chain, while a semi-rough or short-chain type contains only one Ochain repeating unit attached to the core oligosaccharide-lipid A

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B) FACS analysis of BODIPY 493/503 fluorescent intensity in RCC4 cells transfected with siACSL3 or non-targeting control and cultured in media supplemented with FBS or FBS and 100uM Oleic Acid