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Western blot analysis Western blotting was performed as previously described via the following antibodies: FBXO22, BACH1, KLF4, LKB1, SNAL1, PTEN, KDM4A, BAG3, and GAPDH (all from Proteintech, Rosemout, IL)

It remains an investigational compound in Phase 3 clinical development

Microbiome signaling and metabolites are important contributors to barrier regulation

In a 72-week, double-blind phase 2 trial in patients with biopsy-confirmed NASH (legacy term) and fibrosis stage F1F3, once-daily subcutaneous semaglutide produced dose-dependent NASH resolution: 59% at the 0.4 mg dose versus 17% with placebo (a statistically significant difference)