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The difficulty of establishing the efficacy of bronchodilators in this heterogeneous population is further exemplified by the premature termination of a recent major clinical trial (111)

doi: 10.1183/13993003.00176-2022 [DOI] [PubMed] [Google Scholar] 34.Shapiro AJ, Zariwala MA, Ferkol T, et al

Superoxide reporters of the DHE family yield non-specific raw fluorescence, thus quantification must rely on chromatographic separation of DHE2-hydroxyethidium (2-OH-E) by HPLC to measure O 2 and discriminate it from ethidium or other adducts, ideally alongside SOD controls and pathway-selective perturbations (e.g., NOX2 inhibitors, mitochondrial site inhibitors) for source assignment

Disruptions in this axis can lead to increased susceptibility to ferroptosis, highlighting the importance of considering the holistic hepatic environment in disease pathogenesis