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Nov 2008;22(11):4005-4010
2.18 Luteolin in vivo toxicity experiments The C57BL/6 male mice were divided into the DMSO and luteolin (35 M/kg) groups and injected every day
Standard wells and sample wells were loaded with 50 L of serially diluted standards and test samples, respectively, while blank control wells were loaded with 50 L of the sample dilution buffer
Following the removal of the DTT solution, alkylation was performed by incubating the samples with 25 mM iodoacetamide (IAA) in the dark at room temperature for 30 min
