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While glucose has been shown to be almost entirely taken up and metabolized in the neuron, an alternative 13 C tracer is acetate, which has been shown to be preferentially taken up by astrocytes

We found no differences between the preparation conditions tested nor an effect of time to deproteinization on stability of total GSH, suggesting that use of either heparin or EDTA during sample collection can prevent enzymatic degradation of GSH when samples are stored at 80 o C

To maintain freshness and moisture, wrap the ends in a damp paper towel, then place them in a plastic bag and store them upright in the fridge in 2 inches of water, if possible

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