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Activities of NADH-ubiquinone-oxidoreductase (Complex I), succinate-cytochrome c reductase (Complex II/III), and cytochrome c oxidase (Complex IV) were measured by the methods of Birch-Machin and Turnbull ( Assessing protein expression by SDS-PAGE and western blotting Cellular fractions (mitochondria, cytosol, 3050 g protein) from the liver and kidneys of control Wistar and diabetic GK rats were electrophoretically separated by 7.5%12% SDS-PAGE ( Statistical analysis Values are expressed as mean SD of three-five biological replicates

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[DOI] [PMC free article] [PubMed] [Google Scholar] 60.Shin W.H., Jeon M.T., Leem E., Won S.Y., Jeong K.H., Park S.J., McLean C., Lee S.J., Jin B.K., Jung U.J., et al