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The reaction system contains 1 mM CoA, 1 mM NADP + , 1 mM glyoxylate, and excess of purified PanE in 50 mM Tris-HCl buffer (pH = 7.5), which allow the glyoxylate converted to oxalyl-CoA in 30 C

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Notably, autophagy dysfunction and metabolic disturbances form a vicious cycle - impaired autophagic flux exacerbates glucose and lipid metabolic imbalances (Kim and Lee, 2014), which may serve as a key driver for the progressive metabolic deterioration in DM (Figure 3)