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Based on this the use of phosphate buffer, pH 7.4, was suggested by Silk et al to reduce the undesirable non-enzymic hydrolysis to negligible proportions despite of a pH optimum of 8.5 26

Statistical analysis (metabolites) Autoscaling was utilized to normalize data for statistical analysis using MetaboAnalyst 3.0 (available at 27

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Commentary: Ground-glass nodules: The challenge of identifying red flags amidst a background of red herrings
