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In an ideal pH environment, the B12 can release from haptocorrin and bind to an important protein called intrinsic factor
1 g/mL doxycycline (Sigma-Aldrich) was added to the media for 24 h to induce the expression of an shRNA against MYCN in IMR-5/75 cells and overexpress MYCN in SH-SY5Y/MYCN cells
When administered, it rapidly restores vitamin B12 levels, particularly benefiting those with disorders of red blood cell production and nervous system anomalies due to deficiency
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