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His-tagging provides good yields of fusion protein from inexpensive, high capacity resins with moderate purity from E
Treatment starts at 0.25 mg weekly and increases every four weeks through 0.5 mg, 1.0 mg, and 1.7 mg before reaching the maintenance dose of 2.4 mg per week
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Briefly, 5-day-old algal cultures on HS agar medium were cut out along with the agar by using a glass tube, and transferred to separate wells of a 96-well microtiter plates
