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The next day, transfection mixture was prepared using 125 l Mirus LT1 transfection reagent (Mirus Bio, MIR2304), 1.5 ml DMEM (Gibco, 11965-092), 18 g psPax2 (packaging vector), 4 g MD2G (env expression plasmid, VSVG) and 18 g of lentiviral plasmid (sgRNA library)

Finally, glutamate is able to outcompete several other energy substrates, including glucose, lactate, and ketones in astrocytes (McKenna 2012), stressing that glutamate oxidation is a high metabolic priority

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Liver involvement may be suspected in the presence of jaundice, pruritus or abnormal liver function tests