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Materials and Methods Cell lines and culture conditions Certified glioma cell lines U87MG and U343MG (p53 wild-type), U251MG (273Arg-His homozygous p53 mutation) and U138MG (273Arg-His heterozygous p53 mutation) were a kind gift from Bernd Kaina (Mainz, Germany), and were routinely grown in DMEM (Invitrogen, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal calf serum (Cultilab, Campinas, Brazil) and 1% antibioticantimycotic at 37 C in a humidified, 5% CO 2 atmosphere

Non-glucose carbon sources fueling the TCA cycle However, metabolism is not strictly glycolytic, as the tricarboxylic acid (TCA) cycle is functional even under hypoxia [25]

Insect repellents, appropriate clothing, and staying in areas with screens are standard protective measures

doi: 10.1016/j.cell.2006.02.015 58 FacchinSBertinLBonazziELorenzonGDe BarbaCBarberioBet al