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Under high-fat diet conditions, Fat-1 mice exhibit marked metabolic protection, including a reduced risk of non-alcoholic fatty liver disease (NAFLD), attenuated inflammation in brown adipose tissue (BAT), and improved glucose tolerance [25]

Highlights: RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage Easily quantitate GSH levels in live cells using confocal microscopy/FACS Signals from blue and green channels allow for deduction of GSH concentration, given appropriate calibration curve For calibration curve, see: RealThiol (RT) Glutathione (GSH) Calibration Probe Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification
