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Farnesol, a natural sesquiterpenoid, exerts neuroprotective effects against sleep deprivation-induced cognitive impairment by activating the SIRT1/NRF2 signaling pathway

MCF-7 cell lines (ATCC, USA) were cultured in RPMI 1640 media (EuroClone S.p.A., Italy) supplemented with 1% penicillin-streptomycin, 1% L-Glutamine, and 10% fetal bovine serum (FBS) and incubated in a 37 C incubator under 5% CO2 atmosphere

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Combining the two in a single research protocol gives dual-pathway coverage of the regenerative cascade angiogenesis + cell-migration which is closer to the in-vivo process than studying either pathway in isolation