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The subcutaneous injection of GHK-Cu is notoriously associated with localized tissue distress, often referred to colloquially as the copper uglies

Cell viability was confirmed in cells undergoing hypoxia exposure using a commercial viability/cytotoxicity kit (Thermo Fisher, catalog number: R37601)

Financial Support: This work is funded by a National Health and Medical Research Council grant (to C.C.), the University of Queensland (to C.C.), and National Institutes of Health Grant 2RO1CA128799-06A1 (to D.L.)

Because the latter assay is conducted in a cuvette, as opposed to our microtiter plate assay, we also included a third assay, which is based on the 2VP masking in a microtiter plate [11]