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Localization of a substrate binding domain of the human reduced folate carrier to transmembrane domain 11 by radioaffinity labeling and cysteine-substituted accessibility methods

ACS Omega 8(32):2927029280 Rilwan HB, Adebisi SS, Timbuak JA, Oladele SB, Muhammad A, Sadeeq AA, Makena W (2022) Camel milk ameliorates diabetes in pigs by preventing oxidative stress, inflammation and enhancing beta cell function

Propionyl-L-carnitine (200 mg/kg, p.o., drinking water) increases NO synthesis by enhancing eNOS expression in spontaneously hypertensive rats [8]

Trypsin/LysC digestion and streptavidin enrichment Samples were digested by adding 3 l of 100 mM CaCl 2 (final concentration: 1 mM) and 2 g of trypsin/LysC (20 g trypsin/LysC resuspended in 40 l trypsin/LysC buffer, then adding 4 l to each sample), followed by shaking at 37 C for 16 hours