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Briefly, yeast cells were spheroplasted with 500 g ml 1 Zymolyase 100T, homogenized in polyvinylpyrrolidone-buffer (8% polyvinylpyrrolidone, 20 mM potassium phosphate buffer, pH 6.5, 0.75 mM MgCl 2 , and protease and phosphatase inhibitor cocktails) with a Dounce homogenizer

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Tuberculosis serves as a prime example of the significance of bacterial persisters during infection and drugs targeting persisters, as the unique anti-persister drug pyrazinamide (PZA) plays a crucial role in shortening TB therapy and reducing relapse rates

In another 2009 study, Dr