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2-Oxoisocaproic acid is another leucine breakdown product, working alongside the other branched-chain amino acid markers, with processing dependent on B-vitamin-powered enzymes

Electrophysiological recordings further supported this, revealing that Dihexa caused a 1.6-fold increase and Nle-AngIV caused a 1.7-fold increase in the frequency of AMPA-mediated mEPSCs compared to vehicle-treated neurons, indicating an expansion of functional synapses without altering individual synapse properties such as amplitude, rise, or decay times

However, ThT-based aggregation poorly defines the precise nature of the aggregates being monitored and is therefore frequently undertaken in parallel with other techniques [45]