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g Immunoblot and quantification of indicated proteins in AML12 cells pretreated with vehicle or AMPK inhibitor (Bay-3827) in serum-free conditions upon 4 h fatty acids stimuli (n = 3 biological independent samples)

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The membrane was washed with Tris-buffered saline (TBS), containing 0.1% Tween 20 (Beyotime Institute of Biotechnology) three times and was subsequently incubated with horesradish peroxidase-labeled secondary antibody (1:2,000