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Dosing Protocol Standard Research Protocol Dose: 300mcg per day subcutaneous Timing: Fasted ideally 3060 minutes before eating or exercise Injection site: Lower abdomen or thigh (standard subQ technique) Frequency: Daily, or 5 days on / 2 days off Cycle length: 812 weeks, then 46 week break Reconstitution AOD-9604 dissolves readily in bacteriostatic water (BAC water) no acetic acid required, unlike some hydrophobic peptides

Heart rate is also phenotype-coupled: near zero at 1 mg in the obese trial cohort, but +10 bpm or more at the same 1 mg in a leaner body from Phase 1b

But by 2015 and 2016, its effectiveness increased, likely due to higher glucosinolate production from potassium sulfate application

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