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Discoloration: Any yellow, brown, or unusual color is a sign of chemical breakdown

During pyroptosis, GSDMD-N inserts into the cell membrane, forming cytotoxic pores that compromise membrane integrity and facilitate the release of inflammatory mediators (32)

Hydrogen peroxide is also one of the earliest signaling molecules involved in wound-signaling pathways, serving as a local signal for hypersensitive cell death and cell wall stiffening events, and as a mobile signal that stimulates defense-genes in adjacent cells (Orozco-Cardenas et al

Materials and methods Animals Adult (810weekold), male C57BL/6 mice (Jackson Laboratories, Bar Harbor, ME) weighing between 20 and 25 g were used