glutathione reductase system of erythrocytes Erythrocyte metabolism - Chatzinikolaou - 2024 - Acta Physiologica Mathematical model for glutathione dynamics

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However, taking into account the short time spans simulated, it can be expected that alkenes can be further modified, leading to the formation of the observed alcohols after OH-addition 44

Elution with 25 mM Tris-Cl pH 7.4, 500 mM NaCl, and 500 mM Imidazole was followed by TEV cleavage of the His 6 -tag and size exclusion chromatography using Sephacryl S-200 HR 16/600 or 26/600 (Cytiva) equilibrated in 25 mM Tris-Cl pH 7.4, 25 mM NaCl, 1 mM DTT