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[DOI] [PMC free article] [PubMed] [Google Scholar] Zhou FM, Liang Y, Salas R, Zhang L, De Biasi M, Dani JA (2005) Corelease of dopamine and serotonin from striatal dopamine terminals

The results revealed that the levels of pro-inflammatory cytokines IL-6, IL-12, and TNF- secreted by cells treated with NP4 were 1.45, 5, and 1.78 times higher than those secreted by cells treated with NP2, respectively, further confirming the activation of macrophages

Data were blind coded by an experienced researcher, while a second independent researcher randomly coded approximately 20% of the total videos to test inter-observer reliability

The results from in vivo experiments on rats in a maze, monitoring social interactions, locomotor activity, and cage tests, showed that pure asiaticoside, and methanol or ethyl acetate extracts of Centella asiatica had anxiolytic activity