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If none of your protein, or very little, is in the flow-through during your purification, then youve used sufficient glutathione agarose (Figure 5, left)

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The pH scale is logarithmic, which means a change of 1 unit represents a 10 fold change in pH

Methylcobalamin (active form of vitamin B12), 500 mcg per 1 ml glass ampoule, 10 ampoules per pack