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At the same time, reviewers note that evidence for many of AOD9604's proposed effects in humans remains limited and preclinical, and the pivotal human efficacy trial did not confirm the weight-loss signal seen in the earlier, smaller study

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Additional biochemical assays were used to test the ability of compounds to inhibit nicotinamide phosphoribosyl transferase (NAMPT) and NAD + -dependent protein deacetylase sirtuin 1 (SIRT1), two enzymes in the NAD + biosynthesis/salvage pathway
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