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For CCL2 knockdown experiments, HepG2 cells were plated at 1 10 5 cells per well of a 24-well plate and transfected with 20 nM of siRNA against CCL2 (siCCL2, CCL2 Human ON-TARGETplus siRNA, Dharmacon, L-007831-00-000) using Lipofectamine RNAiMAX (Invitrogen, 13778150) in Opti-MEM reduced-serum medium (Gibco) in accordance with the manufacturers protocol

Study 3: Induces collateral blood vessel formation rescuing ischemic tissues

The occurrence of vascular bundles in the petiole of Cucumis melo is almost a closed crescent or circle 99

The remaining part of Cbl (around 70 -80%) is bound to haptocorrin and is called holohaptocorrin ( holoHC )
