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based on consistent formation of -helical structure in initial rounds of structure calculations in which no hydrogen bond restraints were defined), inter-molecular PRE restraints (4 non-interacting copies of the spin label to account for different spin label orientations), inter- and intra-molecular NOE restraints, and ambiguous distance restraints for residues showing CSPs upon binding

Left untreated, bowel obstruction can lead to bowel ischemia, perforation, and other life-threatening complications

We applied bleaching correction between frames to compensate for switching fatigue

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