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Mikitsh JL, Chacko AM

The samples were incubated again for 1 h at 37C in a shaking incubator

(2017), "Therapeutic potential of pro-angiogenic BPC157 is associated with VEGFR2 activation and up-regulation," Journal of Molecular Medicine (Berlin) 95(3):323-333 [PubMed]

Immediately after fixation the samples were washed once briefly with MilliQ and stained overnight at 37C with fresh SA--GAL solution (pH 6.0), containing 2,5 mM Na2HPO4, 7,4 mM Citric Acid, 0.15M NaCl, 2mM MgCl2, 5 mM Potassium Ferricyanide, 5 mM Potassium Ferrocyanide and 25ul/ml 4% X-gal in DMF
