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Overview Duchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene, which encodes dystrophin, a protein normally expressed in skeletal muscle

Purification of GST-tagged fusion proteins Glutathione affinity purification, like many other types of affinity purifications, involves three key steps: 1) binding the target protein to the beads, 2) washing away contaminating proteins, and 3) eluting your protein of interest (Figure 3)

Usually, the next step is to use mass spectrometry to identify those interacting proteins (Kassa et al, 2023)

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