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Therefore, -Glu-Glu does not really behave as a conventional partial agonist as it does not elicit any blocking action on glutamate-evoked responses

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After incubation at 37 C with shaking (500 rpm) for 1 h., the cells were pelleted at 5,000 x g for 10 min at 4 C, washed once with ice-cold phosphate-buffered saline (PBS, pH 7.4), resuspended in 200 L of cold TE lysis buffer (30 mM TrisHCl, 1 mM EDTA, pH 8.0), and transferred to Lysing matrix B tubes (MP Biomedical)

2007 Mar;71(3):397-404 273