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After equilibration, the trypsin digestion solution was added to the Tip and centrifuged at 2000 g for 5 min at 4 C

10.1096/fj.202200597R 37 TardioloG.BramantiP.MazzonE

These compounds ( 14 , 15 , 16 , 18 , and 19 ), were a series of related metabolites that eluted as a series of peaks, each differing in m/z value by +31.97 when analyzed by MS

Maybe they have enough antibodies produced with one shot
