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The slides were heated in sodium citrate antigen retrieval solution (SolarbioBio, C1032) at 95 C for 20 min, 3% hydrogen peroxide for 10 min, and 10% goat serum for 60 min at room temperature
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This in turn allows trans-homodimerization of claudin-2 to form a paracellular cation channel that promotes pore pathway permeability
