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It is required for mitochondrial energy metabolism, normal nervous system function, red blood cell development and maturation, DNA synthesis and the methylation cycle, and has antioxidant effects at the cellular level

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Certain populations face higher deficiency risk: vegetarians and vegans (B12 is primarily found in animal products), adults over 50 (decreased stomach acid reduces B12 absorption), individuals with digestive disorders like Crohn's disease or celiac disease, and those taking medications like metformin or proton pump inhibitors

In cultured hippocampal neurons, Dihexa treatment for 5 days induced a near 3-fold increase in the number of actin-enriched spines by 41 spines/50m for Dihexa vs