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Description
This experiment independently confirmed CRL4 DCAF16 and SCF FBXO3 as being responsible for the activity of dHTC2 and dHTC3, respectively
Water and Trifluoroacetic Acid (TFA): Peptides are often lyophilized (freeze-dried) with acids like TFA to make them stable as a powder
These molecules typically target bacterial cell membranes, causing membrane disruption and bacterial death
Samples (10 g in triplicate) were harvested on days 0, 1, 3, 5, and 7 and washed twice with 50 mM TrisHCl (pH = 7)10 mM EDTA for 20 min
