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In erythrocyte, plasma, whole-blood, PBMC, splenocyte, or other blood-derived systems, peptide purity, residual TFA or other counter-ions, pH, osmolarity, residual solvents, endotoxin, aggregation, storage history, and freeze-thaw exposure can affect the readout

The shuttle vectors contain a 1000 bp 5 flank consisting of the F11 or B8 promoter and a 3 flank consisting of 1000 bp of wt viral sequence 3 of the viral F11 or B8 protein coding sequence, as well as, outside of the insertion site flanking sequences, a green fluorescent protein (GFP) expression cassette for selection

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Although it was fully corroborated that such extract lacks any known natural inhibitor of mTOR to date, the metabolite or metabolites present in such active fraction completely inhibited mTORC1 and controlled Akt activation by blocking mTORC2 phosphorylation at Ser2481