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The plasma samples were diluted 100 and the assay conducted as per the manufacturers instruction

The resulting -hydroxy-1, N 2 -propanoguanine-modified oligodeoxynucleotide was mixed at a 1:1 molar ratio with the complementary oligo: 5-[phos]-CCC TCT TCC GCT CTT CTT TC-3 in PBS and annealed at 85 C for 5 min, ramped to 25 C at 0.1 C s-1 and flash frozen immediately to prevent crosslink formation

Other trials have found no measurable melanin change at all

[DOI] [PubMed] [Google Scholar] (104).Sklar JG, Wu T, Kahne D, and Silhavy TJ (2007) Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli