igf-1 lr3 minimize side effects harm reduction Late-life targeting of the receptor improves healthspan and lifespan in female mice IGF-1 promotes cell surface expression
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It's about building good lab habits that respect the science and the investment you've made in your research materials

Cell immunofluorescence The 24-well plates were carefully wetted with phosphate buffered saline (PBS) to remove excess liquid
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Collagenase solution was added and shaking was performed on a shaker for 3 min at 1700 rpm, followed by incubation in a 37 C water bath for 5 min, alternating 10 times
