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Mice exposed to 30 min MCAo were transcardially perfused in deep anesthesia at 72 h after reperfusion using 0.1 M 0.1 M pH 7.4 phosphate-buffered saline (PBS) followed by 4% paraformaldehyde (PFA) in 0.1 M pH 7.4 PBS

The study adhered to the principles of the Declaration of Helsinki and was approved by the Institutional Ethics Committee of the Eye and Ears, Nose, and Throat Hospital, Fudan University (No.2024215)