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Enriched gene functions and canonical biological pathways were identified with Ingenuity Pathways Analysis software (Ingenuity Systems, Redwood City, CA, USA) using Fishers exact test

551 NGS of nuclear genes involved in mtDNA maintenance, mitochondrial function, and metabolism using targeted gene panels and whole exome sequencing is a preferred approach

Adding to this trend of third party access to energy data has been the broader data sharing initiatives in the space over the past 5 years

In addition, mouse IgG from serum (Sigma-Aldrich, I5381) was coated in parallel (0.25 g/mL) as a positive control for the detection antibody used