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After quantifying the total protein in the tissue samples, 20 g of protein samples were diluted in 2 Laemmli sample buffer supplemented with -mercaptoethanol in a ratio of 950:50 L

PARG inhibition facilitates BRD4MZ1CRL2 VHL ternary complex formation and K29/K48-branched ubiquitylation Having shown that PARG inhibition by PDD robustly promotes PROTAC-induced degradation of BRD4, we next investigated whether the action of PDD is exerted before or after the ubiquitylation step

N Engl J Med 387(5):408420

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