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The lipid solutions were dried by a nitrogen stream and hydrated with 10 mm Tris-HCl, 100 mm NaCl, and pH 8 buffer to a lipid-phosphate concentration of ~ 20 mm

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LRO biogenetic genes are required for preconditioning-induced tolerance to BA toxicity We have previously shown that BA preconditioning confers protection against a lethal BA exposure by engaging a daf-16- and skn-1 -dependent stress- and detoxification response 26