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The cells were first collected by centrifugation at 12,000 g for 5 min, resuspended in RNA later Solution (Ambion), and harvested from the RNA later suspension by centrifugation after overnight storage at 4C

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Food Sci Nutr 12(9):61576173

Basic research describes experimental or theoretical work undertaken primarily to acquire new knowledge of the underlying foundations of phenomena and observable facts, without any particular application or use in view. Applied research is also original investigation in service of acquiring new knowledge, but is directed primarily towards a specific, practical aim or objective. Development, meanwhile, involves applying ideas formed from research and turning them into either new products or improvements to existing products