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Description
Results Spectroscopic characterization of T94 2.61 I/E134 3.49 Q, T94 2.61 I/M257 6.40 Y, E134 3.49 Q/M257 6.40 Y and T94 2.61 I/E134 3.49 Q/M257 6.40 Y mutants We expressed the recombinant opsins corresponding to double and triple mutants combining individual point mutations T94 2.61 I, E134 3.49 Q and M257 6.40 Y in COS-1 cells and immunopurified with the Rho-1D4 antibody in 2 mM Na 2 HPO 4 , pH 6.0, 0.05% DDM (see Materials and Methods section)

10.1016/0014-5793(76)80112-3 9 CunninghamL

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